Generate the SAV Indexing Table
This application writes out index summary metrics similar to the SAV indexing tab.
Running the Program
The program runs as follows:
$ index-summary 11115124_11854Unbin2R2I
In this sample, 11115124_11854Unbin2R2I is a run folder and the summary is written to the standard output as follows
# Version: v1.0.4-224-gacc6c8e Lane 1 Total Reads PF Reads % Read Identified (PF) CV Min Max 22855008 19391826 96.7232 0.5136 0.0001 2.9831 Index Number Sample Id Project Index 1 (I7) Index 2 (I5) % Read Identified (PF) 1 AF_1-5_spike NA AAGAGGCA AAGGAGTA 1.2232 2 WU_1_spike NA AAGAGGCA ACTGCATA 1.5316 ...
The InterOp sub folder may contain any of the following files:
- IndexMetricsOut.bin
- TileMetricsOut.bin
The run folder should have:
- RunInfo.xml
- RunParameters.xml (This is optional for later platforms)
Error Handling
The index-summary
program will print an error to the error stream and return an error code (any number except 0) when an error occurs. There are two likely errors that may arise:
1. The InterOp path was incorrect 2. The InterOp files do not contain valid data
A missing InterOp file will be silently ignored. Incomplete InterOp files are also ignored.