% >= Q30

The QMetricsOut.bin InterOp file contains a histogram of the counts of PF clusters at each quality value ranging from 1 to 50 for each lane, tile, and cycle. % >= Q30 is calculated as the sum of the populations in bins with a quality value of 30 or greater divided by the total non-N basecalls (sum of the population over all bins times 100). Note that we perform these calculations on usable cycles only (in other words, we exclude the last cycle of each read).

See also

illumina::interop::unittest::q_metric_requirements

Lane

For all tiles over all lanes for a given read, for all usable cycles (i.e. not counting the last cycle of the read), the sum of the populations in bins with a quality value of 30 or greater divided by the sum of the population over all bins times 100.

Read

For all tiles in a lane for a given read, for all usable cycles (i.e. not counting the last cycle of the read), the sum of the populations in bins with a quality value of 30 or greater divided by the sum of the population over all bins times 100.

Non-Indexed Total

For all tiles over all non-indexed reads, for all usable cycles (i.e. not counting the last cycle of each read), the sum of the populations in bins with a quality value of 30 or greater divided by the sum of the population over all bins times 100.

Total

For all tiles over all reads, for all usable cycles (i.e. not counting the last cycle of each read), the sum of the populations in bins with a quality value of 30 or greater divided by the sum of the population over all bins times 100.